Cell-free Protein Synthesis
With its detailed description of membrane protein expression, high-throughput and genomic-scale expression studies, both on the analytical and the preparative scale, this book covers the latest advances in the field. The step-by-step protocols and practical examples given for each method constitute practical advice for beginners and experts alike.effects of folding ligands 23 a post-translational 38, 175 a temperature effects 21 protein kinases 38, 51, 162f. protein library ... 98 self-inhibition phenomenon 8, 11 semi-continuous flow system (SCCF) 205 sequential cell-free protein synthesis ... split-primer PCR, see polymerase chain reaction (PCR) SP6 promoter 116, 118f. stereo-array isotope labeling (SAIL) 92 ... conceptual diagram 219 structural genomics (SG) 52, 82, 194 structural proteomics 97ff, 106 sucrose gradient analysisanbsp;...
| Title | : | Cell-free Protein Synthesis |
| Author | : | Alexander S. Spirin, James R. Swartz |
| Publisher | : | John Wiley & Sons - 2008 |
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